Lizard tails give clues to cartilage formation (2024)

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August 22, 2023

At a Glance

  • Scientists identified cells that play key roles in cartilage creation and the regrowth of severed tails in a small lizard.
  • The findings add to our understanding of tissue regeneration and may point to new avenues for repairing damaged cartilage in humans.
Lizard tails give clues to cartilage formation (1)

Researchers gained insights into how lizards like this green anole grow back their tails.Steve Bower / Shutterstock

Many lizards, such as the green anole, have the ability to detach and regrow their tails, which helps them escape the grasp of predators. But the new tail’s main structural component is made of cartilage rather than the bone that was in the original tail. The regrown lizard tail also includes tissues like muscle, nerves, and blood vessels.

Scientists have been studying the lizard’s unique ability to regenerate its tail in hopes that it can offer new insights into cartilage growth or limb repair in people. Most mammals, including humans, respond to tissue injury or limb loss by forming fibrous or scar tissues instead of activating a regenerative process. Human adults are not able to naturally heal or regrow damaged cartilage.

To learn more about cartilage creation, a research team led by Dr. Thomas Lozito at the University of Southern California examined cellular and molecular details of limb regeneration in the anole lizard. They used a technique called single-cell RNA sequencing to determine which types of cells were present at different time points after tail loss in lizards, and which types of genes were activated. Their results were reported in Nature Communications on August 10, 2023.

The scientists gained new insights into two types of cells, called fibroblasts and phagocytes, that are essential to forming new cartilage in the regrowing tail. Fibroblasts are a type of connective tissue cell. They make and secrete collagen and other proteins that help to maintain the structure of tissues. Analyses revealed different subsets of fibroblasts that played different roles in forming cartilage during the 28 days after tail loss.

Lizard tails give clues to cartilage formation (2)

Microscope image shows proteins active during lizard tail regeneration.Ariel Vonk / Lozito Lab

Phagocytes are a type of immune cell that protects the body by gobbling up bacteria, foreign particles, and dead or dying cells. Phagocytes and other immune cells are known to congregate at injured sites. Tail regrowth involved distinct locations for different phagocytes. Factors secreted by certain phagocytes proved critical for signaling fibroblasts to build new cartilage. One particular type of phagocyte, called a septoclast, was especially important for regrowing lizard tails.

To clarify the role of septoclasts, the researchers isolated these cells from lizard tails and transferred the factors they secreted into lizards that had an amputated leg. Lizard legs, like mammal limbs, do not naturally regrow when injured. Rather, they tend to form fibrous and scar tissues, which inhibit regrowth. The scientists found that factors from septoclasts could suppress scarring in severed lizard limbs and enable formation of new cartilage.

The researchers also identified a well-known signaling pathway, called Hedgehog, that is crucial for cartilage formation. Lizards treated with a drug that blocks Hedgehog regrew tails that were normal length but lacked cartilage.

“Those two cell types working together laid the foundation for the beginning of the regenerative process,” Lozito says. “This represents an important step, because we need to understand the process in great detail before we can try to recreate it in mammals.”

—by Vicki Contie

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References:Single-cell analysis of lizard blastema fibroblasts reveals phagocyte-dependent activation of Hedgehog-responsive chondrogenesis. Vonk AC, Zhao X, Pan Z, Hudnall ML, Oakes CG, Lopez GA, Hasel-Kolossa SC, Kuncz AWC, Sengelmann SB, Gamble DJ, Lozito TP. Nat Commun. 2023 Aug 10;14(1):4489. doi: 10.1038/s41467-023-40206-z. PMID: 37563130.

Funding:NIH’s National Institute of General Medical Sciences (NIGMS) and National Cancer Institute (NCI).

Lizard tails give clues to cartilage formation (2024)

FAQs

Lizard tails give clues to cartilage formation? ›

Lizard tails give clues to cartilage formation

Do lizards have cartilage? ›

Lizards, however, represent an organism group that spontaneously generates an abundance of cartilage in response to skeletal injury. There are generally two mechanisms by which skeletal tissues are regenerated in adult organisms: fracture healing and blastema-based regeneration.

What do tails do for lizards? ›

Since the tail is an important organ of lizards for survival as it helps them nourish, run, leap, mate, and escape the next predator, losing the tail is a costly sacrifice. Therefore, in normal circ*mstances, lizards retain their tails sturdily fixed to the body.

What are the functions of tails in reptiles? ›

Lizard tails serve numerous purposes. They aide in balance and locomotion, maintain social status, and are a body area for fat storage. The tail provides a food source during periods of starvation and reproduction.

Does a lizards tail have a bone? ›

To get out of sticky situations, some lizards detach their tails from their bodies and scurry away. Scientists have long wondered how the bones and muscles in these tails—which help with balance and movement—can sever with ease when needed, but stay firmly in place when not.

Do reptiles have cartilage instead of bones? ›

Reptiles, including turtles, snakes, and lizards, have a fully ossified skeleton, which means their skeleton is completely made of bone.

How do lizard tails regenerate? ›

Lizards can regrow severed tails, making them the closest relative to humans that can regenerate a lost appendage. But in lieu of the original tail that includes a spinal column and nerves, the replacement structure is an imperfect cartilage tube.

What is the function of the tail? ›

They provide a source of locomotion for fish and some other forms of marine life. Many land animals use their tails to brush away flies and other biting insects. Most canines use their tails to communicate mood and intention.

Why does a lizard's tail still move when it falls off? ›

When a lizard detaches its tail, the tail whips around and wiggles on the ground. Nerves from the lizard's body are still firing and communicating with each other. In fact, sometimes the tail will keep moving for upwards of a half hour. This distracts a predator and gives the lizard plenty of time to escape.

Why are tails beneficial? ›

Whether they belong to reptiles, insects, birds or mammals, tails serve a wide variety of purposes. Modern animals use their tails for everything from balance to communication and finding mates.

Is it painful for a lizard to lose its tail? ›

It's hard to know if a lizard is intentionally trying to detach its tail, but it often appears so. Losing the tail does not seriously harm a lizard, and may save its life, but the loss of a tail might have some negative effects besides a loss of stored energy.

What happens when you cut a lizard's tail off? ›

The biggest risk to a lizard after tail loss is infection. Debris can get impacted on the tail stump which will introduce bacteria into the lizard's body. The best thing you can do for your tail-less lizard is to keep their habitat very clean.

Do lizard tails have nerves? ›

The regrown lizard tail also includes tissues like muscle, nerves, and blood vessels. Scientists have been studying the lizard's unique ability to regenerate its tail in hopes that it can offer new insights into cartilage growth or limb repair in people.

Are lizards teeth sharp? ›

Varanoid lizards typically have very sharp unicuspid teeth that are subconical to flattened and slightly recurved (e.g., McDowell and Bogert, 1954) (Fig. 8.4). These are often quite snakelike in appearance.

Do geckos have cartilage? ›

Geckos' ability to locomotion in complex habitats is largely due to the particular morphology of their spine. The concave amphicoelous vertebral structure with the persistent notochord and the presence of notochord-derived cartilage enhance flexibility for a greater range of motion.

Do animals have cartilage? ›

Cartilage is an extremely elastic tissue. It is strong and flexible, not rigid and mineralized like bone. Most vertebrate animals have some cartilage. In humans, it's found throughout your body in your joints and even between your boney vertebrae.

Does it hurt when lizards lose their tails? ›

It's hard to know if a lizard is intentionally trying to detach its tail, but it often appears so. Losing the tail does not seriously harm a lizard, and may save its life, but the loss of a tail might have some negative effects besides a loss of stored energy.

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